Patent granted in India to JENNEWEIN BIOTECHNOLOGIE GMBH
Patent number 374974 has been granted to JENNEWEIN BIOTECHNOLOGIE GMBH that is titled PROCESS FOR PURIFICATION OF A NEUTRAL HUMAN MILK OLIGOSACCHARIDE USING SIMULATED MOVING BED CHROMATOGRAPHY. The patent applicant responded to this objection by stating that amended claims of the present invention are a novel process and not a mere use of known process. This patent falls under the category of Chemical. Overall, this patent covers a process for purification of a neutral human milk oligosaccharide in a continuous chromatography from a crude solution comprising the neutral human milk oligosaccharide and contaminants, wherein the crude solution comprising the neutral human milk oligosaccharide and contaminants comprises or consists of a solution which is selected from the group consisting of microbial fermentation extract, biocatalysis reaction solution, chemical synthesis solution, and combinations thereof, wherein the purity of the neutral human milk oligosaccharide in the solution is <80 %, characterized in that the crude solution is applied to at least one purification step using simulated moving bed chromatography, wherein a purified solution comprising the neutral human milk oligosaccharide with a purity of 80 % is provided and wherein the purified solution is spray-dried, and neutral HMO is selected from the group consisting of 2’- fucosyllactose, 3-fucosyllactose, 2’,3-difucosyllactose, lacto-N-triose II, lacto-Ntetraose, lacto-N-neotetraose, lacto-N-fucopentaose I, lacto-N-neofucopentaose I, lacto-N-fucopentaose II, lacto-N-fucopentaose III, lacto-N-fucopentaose V, lactoN-neofucopentaose V, lacto-N-difucohexaose I, lacto-N-difucohexaose II, 6’- galactosyllactose, 3’-galactosyllactose, lacto-N-hexaose, lacto-N-neohexaose, para-lacto-N-hexaose, para-lacto-N-neohexaose and difucosyl-lacto-N-neohexaose, most preferably, the neutral HMO is 2´-fucosyllactose or lacto-N-tetraose.