Patent number 371434 has been granted to VIB VZW, UNIVERSITEIT GENT & WETENSCHAPPELIJK INSTITUUT VOLKSGEZONDHEID that is titled MYCOBACTERIUM MUTANTS FOR VACCINES WITH IMPROVED PROTECTIVE EFFICACY. During the patent prosecution, the patent examiner objected to this patent under section 3(d) of the Indian Patents Act. The patent applicant responded to this objection by stating that the mutation in the SapM gene of Mycobacterium which is a member of the Mycobacterium tuberculosis complex, led to vaccines with improved vaccine efficacy. This patent falls under the category of Traditional Knowledge Biotechnology. Overall, this patent covers mutation of mycobacterium components for vaccine purposes. Vaccines with BCG mutant in genes coding for ManLAM capping a-l, 2-mannosyl transferases and the PI3P phosphatase SapM evaluated as TB vaccines showed significantly longer survival than the parent BCG.